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m tuberculosis strain erdman atcc 35801 ncbi database  (ATCC)


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    ATCC m tuberculosis strain erdman atcc 35801 ncbi database
    M Tuberculosis Strain Erdman Atcc 35801 Ncbi Database, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strain+erdman+atcc+atcc+35801+mycobacterium+tuberculosis/Mycobacterium+tuberculosis+(Zopf)+Lehmann+and+Neumann/pm36642182-244-1-5
    Average 95 stars, based on 388 article reviews
    m tuberculosis strain erdman atcc 35801 ncbi database - by Bioz Stars, 2026-10
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    Virus:

    Article Title: Integration of multi-modal measurements identifies critical mechanisms of tuberculosis drug action.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Mycobacterium tuberculosis: Strain Erdman ATCC ATCC 35801 Mycobacterium tuberculosis: Strain H37Rv ATCC ATCC 25618 Chemicals, peptides, and recombinant proteins bedaquiline (morphology) Asta Tech 43211 bedaquiline (RNA-seq) SigmaAldrich ATE517247923 clofazimine SigmaAldrich C8895 ethambutol (morphology) Alfa Aesar J60695 ethambutol (RNA-seq) SigmaAldrich E4630 isoniazid SigmaAldrich I3377 KRS (lysyl-tRNA synthetase inhibitor) University of Dundee Gift linezolid (morphology) Apex Bio A5181 linezolid (RNA-seq) SigmaAldrich PZ0014 moxifloxacin (morphology) Alfa Aesar J66626 moxifloxacin (RNA-seq) SigmaAldrich SML1581 pretomanid Apex Bio A1736 pyrazinamide (morphology) TCI Chemicals P0633 pyrazinamide (RNA-seq) SigmaAldrich PHR1576 rifapentine (morphology) Apex Bio B2127 rifapentine (RNA-seq) SigmaAldrich R0533 rifampicin (morphology) TCI Chemicals R0079 rifampicin (RNA-seq) SigmaAldrich R3501 tbaj-876 TB Alliance Gift tba-7371 TB Alliance Gift tbi-223 TB Alliance Gift gsk-839 GSK Gift gsk-286 GSK Gift sanfetrinem GSK Gift au1235 MedChemExpress HY-101867 actinomycin D Fisher Scientific AAJ60148LB0 amikacin SigmaAldrich A3650 ampicillin SigmaAldrich A9393 bm212 MedChemExpress HY-100725 chloramphenicol SigmaAldrich C0378 capreomycin Fisher Scientific ICN15492483 CCCP SigmaAldrich C2759 cerulenin SigmaAldrich C2389-10mg ciprofloxacin SigmaAldrich phr1044-1g clarithromycin SigmaAldrich A3487-100MG cefotaxime SigmaAldrich C7039 cycloserine Fisher Scientific 239831 daunorubicin SigmaAldrich 30450-5MG delamanid Advanced ChemBlocksInc L13485 doxycycline SigmaAldrich D9891 ethionamide TCI Chemicals E0695 (Continued on next page) Cell Systems 16, 101348, August 20, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER fidaxomicin Fisher Scientific 50-101-5249 gentamicin SigmaAldrich G1264 imipenem SigmaAldrich PHR1796-200MG kanamycin VWR 408 levofloxacin SigmaAldrich 28266 meropenem SigmaAldrich 1392454 mitomycin SigmaAldrich Y0000378 nigericin SigmaAldrich N7143-10MG ofloxacin SigmaAldrich 8757 quabodepistat Otsuka Gift pseudouridimycin Fisher Scientific AG-CN2-0316-M005 SQ109 Fisher Scientific 50-186-7024 streptomycin SigmaAldrich S6501 tetracycline SigmaAldrich 87128 thioridazine Enzo life sciences BMLNS835-0005 orlistat Enzo life sciences ALX-350-152-M250 vancomycin SigmaAldrich V0045000 verapamil Enzo life sciences ALX-55–306-G001 viomycin Fisher Scientific 37-871-0 Critical commercial assays BacTiter-GloTM Microbial Cell Viability Assay Promega G8231 Qiagen RNeasy Mini Kit Qiagen 74104 Deposited data Morphological and RNA-seq data This study https://doi.org/10.17632/7d4m532n7r.1 Data for figure generation This study https://data.mendeley.com/preview/ 7d4m532n7r?a=103a12cb-7012-4531a4d6-9b8d313e7b0d Raw RNA sequencing data This study GSE267556 Recombinant DNA pMV306hsp+LuxG13 Andreu et al. 70 http://n2t.net/addgene:26161; RRID: Addgene_26161 pSE100-recA::’GFP This paper N/A pGMCK-T10M-P606-mmpL3-F644LTsE5-bc GenScript N/A Software and algorithms DECIPHAER modelling This study https://doi.org/10.5281/zenodo.15627527 R N/A https://cran.r-project.org/ MATLAB N/A https://www.mathworks.com/products/ matlab.html python3 N/A https://www.python.org/downloads/ tidyverse(R package) Wickham and RStudio 71 https://cran.r-project.org/web/packages/ tidyverse/index.html ggplot2(R package) Wickham et al. 72 https://cran.r-project.org/web/packages/ ggplot2/index.html ggpubr(R package) Kassambara et al. 73 https://cran.r-project.org/web/packages/ ggpubr/index.html readxls(R package) Wickham et al. 74 https://cran.r-project.org/web/packages/ readxl/index.html stats(R package) N/A https://cran.r-project.org/ umap(R package) Konopka 75 https://cran.r-project.org/web/packages/ umap/index.html (Continued on next page) e2 Cell Systems 16, 101348, August 20, 2025

    Recombinant:

    Article Title: Integration of multi-modal measurements identifies critical mechanisms of tuberculosis drug action.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Mycobacterium tuberculosis: Strain Erdman ATCC ATCC 35801 Mycobacterium tuberculosis: Strain H37Rv ATCC ATCC 25618 Chemicals, peptides, and recombinant proteins bedaquiline (morphology) Asta Tech 43211 bedaquiline (RNA-seq) SigmaAldrich ATE517247923 clofazimine SigmaAldrich C8895 ethambutol (morphology) Alfa Aesar J60695 ethambutol (RNA-seq) SigmaAldrich E4630 isoniazid SigmaAldrich I3377 KRS (lysyl-tRNA synthetase inhibitor) University of Dundee Gift linezolid (morphology) Apex Bio A5181 linezolid (RNA-seq) SigmaAldrich PZ0014 moxifloxacin (morphology) Alfa Aesar J66626 moxifloxacin (RNA-seq) SigmaAldrich SML1581 pretomanid Apex Bio A1736 pyrazinamide (morphology) TCI Chemicals P0633 pyrazinamide (RNA-seq) SigmaAldrich PHR1576 rifapentine (morphology) Apex Bio B2127 rifapentine (RNA-seq) SigmaAldrich R0533 rifampicin (morphology) TCI Chemicals R0079 rifampicin (RNA-seq) SigmaAldrich R3501 tbaj-876 TB Alliance Gift tba-7371 TB Alliance Gift tbi-223 TB Alliance Gift gsk-839 GSK Gift gsk-286 GSK Gift sanfetrinem GSK Gift au1235 MedChemExpress HY-101867 actinomycin D Fisher Scientific AAJ60148LB0 amikacin SigmaAldrich A3650 ampicillin SigmaAldrich A9393 bm212 MedChemExpress HY-100725 chloramphenicol SigmaAldrich C0378 capreomycin Fisher Scientific ICN15492483 CCCP SigmaAldrich C2759 cerulenin SigmaAldrich C2389-10mg ciprofloxacin SigmaAldrich phr1044-1g clarithromycin SigmaAldrich A3487-100MG cefotaxime SigmaAldrich C7039 cycloserine Fisher Scientific 239831 daunorubicin SigmaAldrich 30450-5MG delamanid Advanced ChemBlocksInc L13485 doxycycline SigmaAldrich D9891 ethionamide TCI Chemicals E0695 (Continued on next page) Cell Systems 16, 101348, August 20, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER fidaxomicin Fisher Scientific 50-101-5249 gentamicin SigmaAldrich G1264 imipenem SigmaAldrich PHR1796-200MG kanamycin VWR 408 levofloxacin SigmaAldrich 28266 meropenem SigmaAldrich 1392454 mitomycin SigmaAldrich Y0000378 nigericin SigmaAldrich N7143-10MG ofloxacin SigmaAldrich 8757 quabodepistat Otsuka Gift pseudouridimycin Fisher Scientific AG-CN2-0316-M005 SQ109 Fisher Scientific 50-186-7024 streptomycin SigmaAldrich S6501 tetracycline SigmaAldrich 87128 thioridazine Enzo life sciences BMLNS835-0005 orlistat Enzo life sciences ALX-350-152-M250 vancomycin SigmaAldrich V0045000 verapamil Enzo life sciences ALX-55–306-G001 viomycin Fisher Scientific 37-871-0 Critical commercial assays BacTiter-GloTM Microbial Cell Viability Assay Promega G8231 Qiagen RNeasy Mini Kit Qiagen 74104 Deposited data Morphological and RNA-seq data This study https://doi.org/10.17632/7d4m532n7r.1 Data for figure generation This study https://data.mendeley.com/preview/ 7d4m532n7r?a=103a12cb-7012-4531a4d6-9b8d313e7b0d Raw RNA sequencing data This study GSE267556 Recombinant DNA pMV306hsp+LuxG13 Andreu et al. 70 http://n2t.net/addgene:26161; RRID: Addgene_26161 pSE100-recA::’GFP This paper N/A pGMCK-T10M-P606-mmpL3-F644LTsE5-bc GenScript N/A Software and algorithms DECIPHAER modelling This study https://doi.org/10.5281/zenodo.15627527 R N/A https://cran.r-project.org/ MATLAB N/A https://www.mathworks.com/products/ matlab.html python3 N/A https://www.python.org/downloads/ tidyverse(R package) Wickham and RStudio 71 https://cran.r-project.org/web/packages/ tidyverse/index.html ggplot2(R package) Wickham et al. 72 https://cran.r-project.org/web/packages/ ggplot2/index.html ggpubr(R package) Kassambara et al. 73 https://cran.r-project.org/web/packages/ ggpubr/index.html readxls(R package) Wickham et al. 74 https://cran.r-project.org/web/packages/ readxl/index.html stats(R package) N/A https://cran.r-project.org/ umap(R package) Konopka 75 https://cran.r-project.org/web/packages/ umap/index.html (Continued on next page) e2 Cell Systems 16, 101348, August 20, 2025

    RNA Sequencing:

    Article Title: Integration of multi-modal measurements identifies critical mechanisms of tuberculosis drug action.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Mycobacterium tuberculosis: Strain Erdman ATCC ATCC 35801 Mycobacterium tuberculosis: Strain H37Rv ATCC ATCC 25618 Chemicals, peptides, and recombinant proteins bedaquiline (morphology) Asta Tech 43211 bedaquiline (RNA-seq) SigmaAldrich ATE517247923 clofazimine SigmaAldrich C8895 ethambutol (morphology) Alfa Aesar J60695 ethambutol (RNA-seq) SigmaAldrich E4630 isoniazid SigmaAldrich I3377 KRS (lysyl-tRNA synthetase inhibitor) University of Dundee Gift linezolid (morphology) Apex Bio A5181 linezolid (RNA-seq) SigmaAldrich PZ0014 moxifloxacin (morphology) Alfa Aesar J66626 moxifloxacin (RNA-seq) SigmaAldrich SML1581 pretomanid Apex Bio A1736 pyrazinamide (morphology) TCI Chemicals P0633 pyrazinamide (RNA-seq) SigmaAldrich PHR1576 rifapentine (morphology) Apex Bio B2127 rifapentine (RNA-seq) SigmaAldrich R0533 rifampicin (morphology) TCI Chemicals R0079 rifampicin (RNA-seq) SigmaAldrich R3501 tbaj-876 TB Alliance Gift tba-7371 TB Alliance Gift tbi-223 TB Alliance Gift gsk-839 GSK Gift gsk-286 GSK Gift sanfetrinem GSK Gift au1235 MedChemExpress HY-101867 actinomycin D Fisher Scientific AAJ60148LB0 amikacin SigmaAldrich A3650 ampicillin SigmaAldrich A9393 bm212 MedChemExpress HY-100725 chloramphenicol SigmaAldrich C0378 capreomycin Fisher Scientific ICN15492483 CCCP SigmaAldrich C2759 cerulenin SigmaAldrich C2389-10mg ciprofloxacin SigmaAldrich phr1044-1g clarithromycin SigmaAldrich A3487-100MG cefotaxime SigmaAldrich C7039 cycloserine Fisher Scientific 239831 daunorubicin SigmaAldrich 30450-5MG delamanid Advanced ChemBlocksInc L13485 doxycycline SigmaAldrich D9891 ethionamide TCI Chemicals E0695 (Continued on next page) Cell Systems 16, 101348, August 20, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER fidaxomicin Fisher Scientific 50-101-5249 gentamicin SigmaAldrich G1264 imipenem SigmaAldrich PHR1796-200MG kanamycin VWR 408 levofloxacin SigmaAldrich 28266 meropenem SigmaAldrich 1392454 mitomycin SigmaAldrich Y0000378 nigericin SigmaAldrich N7143-10MG ofloxacin SigmaAldrich 8757 quabodepistat Otsuka Gift pseudouridimycin Fisher Scientific AG-CN2-0316-M005 SQ109 Fisher Scientific 50-186-7024 streptomycin SigmaAldrich S6501 tetracycline SigmaAldrich 87128 thioridazine Enzo life sciences BMLNS835-0005 orlistat Enzo life sciences ALX-350-152-M250 vancomycin SigmaAldrich V0045000 verapamil Enzo life sciences ALX-55–306-G001 viomycin Fisher Scientific 37-871-0 Critical commercial assays BacTiter-GloTM Microbial Cell Viability Assay Promega G8231 Qiagen RNeasy Mini Kit Qiagen 74104 Deposited data Morphological and RNA-seq data This study https://doi.org/10.17632/7d4m532n7r.1 Data for figure generation This study https://data.mendeley.com/preview/ 7d4m532n7r?a=103a12cb-7012-4531a4d6-9b8d313e7b0d Raw RNA sequencing data This study GSE267556 Recombinant DNA pMV306hsp+LuxG13 Andreu et al. 70 http://n2t.net/addgene:26161; RRID: Addgene_26161 pSE100-recA::’GFP This paper N/A pGMCK-T10M-P606-mmpL3-F644LTsE5-bc GenScript N/A Software and algorithms DECIPHAER modelling This study https://doi.org/10.5281/zenodo.15627527 R N/A https://cran.r-project.org/ MATLAB N/A https://www.mathworks.com/products/ matlab.html python3 N/A https://www.python.org/downloads/ tidyverse(R package) Wickham and RStudio 71 https://cran.r-project.org/web/packages/ tidyverse/index.html ggplot2(R package) Wickham et al. 72 https://cran.r-project.org/web/packages/ ggplot2/index.html ggpubr(R package) Kassambara et al. 73 https://cran.r-project.org/web/packages/ ggpubr/index.html readxls(R package) Wickham et al. 74 https://cran.r-project.org/web/packages/ readxl/index.html stats(R package) N/A https://cran.r-project.org/ umap(R package) Konopka 75 https://cran.r-project.org/web/packages/ umap/index.html (Continued on next page) e2 Cell Systems 16, 101348, August 20, 2025



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    Mycobacterium tuberculosis folate pathway. Canonical pathway enzyme (green): DHPS: dihydropteroate synthase, DHFS: dihydrofolate synthase, DHFR: dihydrofolate reductase, TS: thymidylate synthase, SHMT: serine hydroxyl methyltransferase; Alternate pathway enzymes (orange) FDTS: flavin-dependent thymidylate synthase, Rv2671

    Journal: Cell chemical biology

    Article Title: Drugging the Folate Pathway in Mycobacterium Tuberculosis : The Role of Multi-Targeting Agents

    doi: 10.1016/j.chembiol.2019.02.013

    Figure Lengend Snippet: Mycobacterium tuberculosis folate pathway. Canonical pathway enzyme (green): DHPS: dihydropteroate synthase, DHFS: dihydrofolate synthase, DHFR: dihydrofolate reductase, TS: thymidylate synthase, SHMT: serine hydroxyl methyltransferase; Alternate pathway enzymes (orange) FDTS: flavin-dependent thymidylate synthase, Rv2671

    Article Snippet: The accession numbers are provided in . table ft1 table-wrap mode="anchored" t5 caption a7 Reagents or Resources Source Identifier Chemicals Beta-nicotinamide adenine dinucleotide phosphate (NADPH) Alfa-Aesar Cat#J60387 Dihydrofolic acid (DHF) Sigma-Aldrich Cat#D7006 Tetrahydrofolic acid (THF) Sigma-Aldrich Cat#T3125 Methotrexate Sigma-Aldrich Cat#A6770 Isoniazid Sigma-Aldrich Cat#I3377 Flavin adenine dinucleotide disodium salt hydrate (FAD) Sigma-Aldrich Cat#F6625 2’-deoxyuridine-5’-monophosphate disodium salt (dUMP) Alfa-Aesar Cat#J64627 Thymidine 5’-monophosphate disodium salt (dTMP) Sigma-Aldrich Cat#T7004 5,10-Methylenetetrahydrofolate (mTHF) Toronto Research Chemicals Cat#F680350 Isopropyl-1-thio-b-D-galacto-pyranoside (IPTG) Thermo Scientific Cat#R0392 para -Aminosalicylic acid Sigma-Aldrich Cat#A79604 Nextera XT DNA library preparation kit Illumina Cat#FC-131 AMPure XP beads Beckman-Coulter Cat#A63880 Microbial Strains Mycobacterium tuberculosis Erdman Strain ATCC Cat#35801 BL21 (DE3) competent E. Coli New England BioLabs Cat#C2527I Recombinant DNA Plasmid pET-28a(+)-MtbRv271 GenScript N/A Plasmid pET-24d-MtbFDTS GenScript N/A Plasmid pET-41a(+)-MtbDHFR GenScript N/A Plasmid pET-41a(+)-HuDHFR GenScript N/A Deposited Data Macromolecular Crystallography Structure of Mtb DHFR bound to UCP1172 This study PDB ID: 6DDP Structure of Mtb DHFR bound to UCP1175 This study PDB ID: 6DDS Structure of Mtb DHFR bound to PAS-M This study PDB ID: 6DDW Structure of Hu DHFR bound to UCP1172 This study PDB ID: 6DE4 Structure of Mtb Rv2671 bound to UCP1063 This study PDB ID: 6DE5 Genome Sequences (BioProject ID: PRJNA523029) Whole Genome Sequence of Wild-type Mtb This paper BioSample accession: SAMN10962817 Whole Genome Sequence of PAS-R folC (E153G) mutant This paper BioSample accession: SAMN10962818 Whole Genome Sequence of PAS-R folC (I43T) mutant This paper BioSample accession: SAMN10962820 Whole Genome Sequence of PAS-R thyX mutant This paper BioSample accession: SAMN10962819 Open in a separate window KEY RESOURCE TABLE

    Techniques:

    Left: Autoradiograph of TLC analysis of lipids extracted from 14C acetate labeled Mtb Erdman cells treated with UPC1172, PAS or DMSO as a control (CTR); Autoradiograph of standard (middle) and argentation (right) TLC analysis of methyl esters of fatty and mycolic acids isolated from 14C acetate labeled Mtb Erdman cells treated with UPC1172, PAS, or DMSO as a control (CTR)

    Journal: Cell chemical biology

    Article Title: Drugging the Folate Pathway in Mycobacterium Tuberculosis : The Role of Multi-Targeting Agents

    doi: 10.1016/j.chembiol.2019.02.013

    Figure Lengend Snippet: Left: Autoradiograph of TLC analysis of lipids extracted from 14C acetate labeled Mtb Erdman cells treated with UPC1172, PAS or DMSO as a control (CTR); Autoradiograph of standard (middle) and argentation (right) TLC analysis of methyl esters of fatty and mycolic acids isolated from 14C acetate labeled Mtb Erdman cells treated with UPC1172, PAS, or DMSO as a control (CTR)

    Article Snippet: The accession numbers are provided in . table ft1 table-wrap mode="anchored" t5 caption a7 Reagents or Resources Source Identifier Chemicals Beta-nicotinamide adenine dinucleotide phosphate (NADPH) Alfa-Aesar Cat#J60387 Dihydrofolic acid (DHF) Sigma-Aldrich Cat#D7006 Tetrahydrofolic acid (THF) Sigma-Aldrich Cat#T3125 Methotrexate Sigma-Aldrich Cat#A6770 Isoniazid Sigma-Aldrich Cat#I3377 Flavin adenine dinucleotide disodium salt hydrate (FAD) Sigma-Aldrich Cat#F6625 2’-deoxyuridine-5’-monophosphate disodium salt (dUMP) Alfa-Aesar Cat#J64627 Thymidine 5’-monophosphate disodium salt (dTMP) Sigma-Aldrich Cat#T7004 5,10-Methylenetetrahydrofolate (mTHF) Toronto Research Chemicals Cat#F680350 Isopropyl-1-thio-b-D-galacto-pyranoside (IPTG) Thermo Scientific Cat#R0392 para -Aminosalicylic acid Sigma-Aldrich Cat#A79604 Nextera XT DNA library preparation kit Illumina Cat#FC-131 AMPure XP beads Beckman-Coulter Cat#A63880 Microbial Strains Mycobacterium tuberculosis Erdman Strain ATCC Cat#35801 BL21 (DE3) competent E. Coli New England BioLabs Cat#C2527I Recombinant DNA Plasmid pET-28a(+)-MtbRv271 GenScript N/A Plasmid pET-24d-MtbFDTS GenScript N/A Plasmid pET-41a(+)-MtbDHFR GenScript N/A Plasmid pET-41a(+)-HuDHFR GenScript N/A Deposited Data Macromolecular Crystallography Structure of Mtb DHFR bound to UCP1172 This study PDB ID: 6DDP Structure of Mtb DHFR bound to UCP1175 This study PDB ID: 6DDS Structure of Mtb DHFR bound to PAS-M This study PDB ID: 6DDW Structure of Hu DHFR bound to UCP1172 This study PDB ID: 6DE4 Structure of Mtb Rv2671 bound to UCP1063 This study PDB ID: 6DE5 Genome Sequences (BioProject ID: PRJNA523029) Whole Genome Sequence of Wild-type Mtb This paper BioSample accession: SAMN10962817 Whole Genome Sequence of PAS-R folC (E153G) mutant This paper BioSample accession: SAMN10962818 Whole Genome Sequence of PAS-R folC (I43T) mutant This paper BioSample accession: SAMN10962820 Whole Genome Sequence of PAS-R thyX mutant This paper BioSample accession: SAMN10962819 Open in a separate window KEY RESOURCE TABLE

    Techniques: Autoradiography, Labeling, Control, Isolation

    KEY RESOURCE TABLE

    Journal: Cell chemical biology

    Article Title: Drugging the Folate Pathway in Mycobacterium Tuberculosis : The Role of Multi-Targeting Agents

    doi: 10.1016/j.chembiol.2019.02.013

    Figure Lengend Snippet: KEY RESOURCE TABLE

    Article Snippet: The accession numbers are provided in . table ft1 table-wrap mode="anchored" t5 caption a7 Reagents or Resources Source Identifier Chemicals Beta-nicotinamide adenine dinucleotide phosphate (NADPH) Alfa-Aesar Cat#J60387 Dihydrofolic acid (DHF) Sigma-Aldrich Cat#D7006 Tetrahydrofolic acid (THF) Sigma-Aldrich Cat#T3125 Methotrexate Sigma-Aldrich Cat#A6770 Isoniazid Sigma-Aldrich Cat#I3377 Flavin adenine dinucleotide disodium salt hydrate (FAD) Sigma-Aldrich Cat#F6625 2’-deoxyuridine-5’-monophosphate disodium salt (dUMP) Alfa-Aesar Cat#J64627 Thymidine 5’-monophosphate disodium salt (dTMP) Sigma-Aldrich Cat#T7004 5,10-Methylenetetrahydrofolate (mTHF) Toronto Research Chemicals Cat#F680350 Isopropyl-1-thio-b-D-galacto-pyranoside (IPTG) Thermo Scientific Cat#R0392 para -Aminosalicylic acid Sigma-Aldrich Cat#A79604 Nextera XT DNA library preparation kit Illumina Cat#FC-131 AMPure XP beads Beckman-Coulter Cat#A63880 Microbial Strains Mycobacterium tuberculosis Erdman Strain ATCC Cat#35801 BL21 (DE3) competent E. Coli New England BioLabs Cat#C2527I Recombinant DNA Plasmid pET-28a(+)-MtbRv271 GenScript N/A Plasmid pET-24d-MtbFDTS GenScript N/A Plasmid pET-41a(+)-MtbDHFR GenScript N/A Plasmid pET-41a(+)-HuDHFR GenScript N/A Deposited Data Macromolecular Crystallography Structure of Mtb DHFR bound to UCP1172 This study PDB ID: 6DDP Structure of Mtb DHFR bound to UCP1175 This study PDB ID: 6DDS Structure of Mtb DHFR bound to PAS-M This study PDB ID: 6DDW Structure of Hu DHFR bound to UCP1172 This study PDB ID: 6DE4 Structure of Mtb Rv2671 bound to UCP1063 This study PDB ID: 6DE5 Genome Sequences (BioProject ID: PRJNA523029) Whole Genome Sequence of Wild-type Mtb This paper BioSample accession: SAMN10962817 Whole Genome Sequence of PAS-R folC (E153G) mutant This paper BioSample accession: SAMN10962818 Whole Genome Sequence of PAS-R folC (I43T) mutant This paper BioSample accession: SAMN10962820 Whole Genome Sequence of PAS-R thyX mutant This paper BioSample accession: SAMN10962819 Open in a separate window KEY RESOURCE TABLE

    Techniques: DNA Library Preparation, Recombinant, Plasmid Preparation, Sequencing, Mutagenesis